Immunofluorescence studies of MPA-treated cells provided evidence for decreased membrane disassembly/reassembly of α-smooth muscle actin and F-actin fibers, which was correlated with sustained quantitative and qualitative modifications of actin-associated proteins: calponin was overexpressed and became associated with actin fibers, whereas phosphorylation levels of cofilin and myosin light chain increased, suggesting both an activation of the mechanisms responsible for actin polymerization and an inhibition of actin-depolymerizing processes. Using the IP15 cell line, we found that treatment with 1 μmol/L MPA inhibited both receptor-dependent (angiotensin II) and receptor-independent (KCl) contractile responses, as well as serum-induced migration activity, suggesting alterations in the intracellular mechanisms that control mesangial cell motility. The aim of this study was to investigate whether mycophenolic acid (MPA) affects cytoskeletal organization and motility of human mesangial cells. Customer Service and Ordering InformationĬytoskeleton alterations are a hallmark of mesangial cell activation during glomerulosclerosis.Stroke: Vascular and Interventional Neurology.
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